---

8.2.57.54 tblish.dataset.Puromycin ¶

Static Method: out = Puromycin () ¶

Reaction Velocity of an Enzymatic Reaction

Description ¶

Reaction velocity versus substrate concentration in an enzymatic reaction involving untreated cells or cells treated with Puromycin.

Format ¶

state

Whether the cell was treated.

conc

Substrate concentrations (ppm).

rate

Instantaneous reaction rates (counts/min/min).

Details ¶

Data on the velocity of an enzymatic reaction were obtained by Treloar (1974). The number of counts per minute of radioactive product from the reaction was measured as a function of substrate concentration in parts per million (ppm) and from these counts the initial rate (or velocity) of the reaction was calculated (counts/min/min). The experiment was conducted once with the enzyme treated with Puromycin, and once with the enzyme untreated.

Source ¶

The data are given in Box & Jenkins (1976). Obtained from the Time Series Data Library at http://www-personal.buseco.monash.edu.au/~hyndman/TSDL/.

References ¶

Bates, D.M. and Watts, D.G. (1988). Nonlinear Regression Analysis and Its Applications. New York: Wiley. Appendix A1.3.

Treloar, M. A. (1974). Effects of Puromycin on Galactosyltransferase in Golgi Membranes. M.Sc. Thesis, U. of Toronto.

Examples ¶

t = tblish.dataset.Puromycin;

# TODO: Port example to Octave